绵羊不同部位脂肪组织microRNA高通量测序及生物信息学分析

doi:10.11843/j.issn.0366-6964.2016.06.003

Abstract

Abstract:

This study aimed to find tissue-specifically expressed miRNAs in ovine adipose tissues.Three small RNA (sRNA) libraries from perirenal，subcutaneous and tail adipose tissues were built for Solexa sequencing.The sequencing results were validated by Stem-loop qRT-PCR technology and further analyzed by bioinformatics methods.The PCR results shown that seven in eight randomly selected miRNAs were consistent with the sequencing results.In perirenal and subcutaneous adipose tissues，128 and 112 conserved miRNAs were obtained，198 and 196 novel miRNAs were found，respectively.Combined with the previous analysis results of miRNAs detected in tail adipose tissue，12 conserved and 66 novel miRNAs were specifically expressed in perirenal adipose tissue；one conserved and 30 novel miRNAs were specifically expressed in subcutaneous adipose tissue；and one conserved and 34 novel miRNAs were specifically expressed in tail adipose tissue.These results provide scientific bases for further research on the regulatory role of miRNA in fat metabolism.

CLC Number:

S813.3
S826

ZHANG Fang，HU Zi-qiao，JING Jiong-jie，PAN Yang-yang，QIAO Li-ying，LI Bao-jun，LIU Jian-hua，LIU Wen-zhong. High-throughput Sequencing and Bioinformatics Analysis on microRNAs Expressed in Adipose Tissues of Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(6): 1093-1101.

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High-throughput Sequencing and Bioinformatics Analysis on microRNAs Expressed in Adipose Tissues of Sheep

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